Development of the phonetic skills in German as the second foreign language on the basis of the English language

The problems of forming phonetic skills of the German language which is studied on the basis of the English language have been considered. The aim of this research is to make the comparative analysis of the phonetic aspects of the foreign languages that are taught one after another. There has been the attempt to analyze, generalize and systematize the material on the given topic which is presented in works in German, English, Ukrainian and Russian on the main theoretical questions connected with the process of teaching the second foreign language. It was shown that while forming phonetic skills in German, it is necessary to give the characteristics to the phonetic, rhythmic and intonation peculiarities of both German and English; to point out the difficulties of mastering the pronunciation system of German, to develop the introductory course and the material for phonetic warming-up and to work out the algorithm of introducing a new sound

Lexical and grammatical means of expressing modality in the german language as one of the aspects of teaching a foreign language while training post-graduate students

The aim of the article is to analyze lexical and grammatical means of expressing modality in the literature on technical sciences in German and the ways of their translating. Basing on the aim of the research, it is necessary to solve the following problems: 1) to distinguish the means of expressing modality in the literature on technical sciences; 2) to define the role of lexical and grammatical means of expressing modality in a special test; 3) to identify the means of expressing modality which are the most difficult to translate and to understand in the test; 4) to give some recommendations as to developing and improving the skills of translating and using the means of expressing modality in speech

e-Course of Theoretical Mechanics

The concept, structure and contents of the Internet textbook on classical mechanics intended for Higher Technical Institutions are presented in this work. Aspects of program realization of textbook applications and the technology of elaborating the textbook in the “Hecadem” Internet-teaching environment are given too

Modelling, simulation and optimisation of pulse-reverse regime of copper, silver and gold electrodeposition

Pulse-reverse power modes are used in galvanotechniques in order to obtain coatings with better characteristics in terms of gloss, adhesion, tracking sharp edges and uniform distribution of deposits on complex shape objects, compared with the coatings produced by constant current modes. Pulse-reverse modes also allow the use of a higher current density, and thus the production speed of electroplating cells increases. Systems for standard electroplating of copper, silver and gold are optimised by the suitable choice of duration and intensity of the pulses. It is shown that coatings with satisfactory quality can be deposited using higher current density, different modes of pulsed current in a very short period of time, without expensive and often dangerous additives in the electrolyte. Parameters of the model for certain electrochemical systems were determined by modelling and computer simulation, so the system behaviour under different circumstances becomes predictive

Electrochemical Behavior of Supercapacitor Electrodes Based on Activated Carbon and Fly Ash

The possibility of applying fly ash from power plants as a binder in supercapacitor electrodes based on activated carbon was investigated in this research. Based on the mechanical and electrical properties of the electrodes, the optimal ratio between fly ash and AC was determined. Supercapacitor electrodes were prepared in two ways: by pressing and by laser solidification. The preparation method significantly affected physical properties of the electrodes as well as the electrochemical behavior in supercapacitor setup. The electrodes were electrochemically tested by galvanostatic and potentiostatic methods and cyclic voltammetry. In order to improve the estimation of supercapacitor parameters, mathematical model that perfectly describes the behavior of investigated electrodes in aqueous solution of sodium nitrate was developed. The best results were obtained with laser-solidified electrode in 1M aqueous solution of NaNO3. Specific capacitance of 105 F/g, serial resistance of 0.57 Omega and self-discharge resistance of 95 Omega were achieved. Stability at high number of cycles proved to be very good. After 2000 cycles of CV at scan rate of 100 mV/s, specific capacitance fell by only 4.6 %

Profiling of 179 miRNA expression in blood plasma of lung cancer patients and cancer-free individuals

Lung cancer is one of major cancers, and survival of lung cancer patients is dictated by the timely detection and diagnosis. Cell-free circulating miRNAs were proposed as candidate biomarkers for lung cancer. These RNAs are frequently deregulated in lung cancer and can persist in bodily fluids for extended periods of time, shielded from degradation by membrane vesicles and biopolymer complexes. To date, several groups reported the presence of lung tumour-specific subsets of miRNAs in blood. Here we describe the profiling of blood plasma miRNAs in lung cancer patients, healthy individuals and endobronchitis patients using miRCURY LNA miRNA qPCR Serum/Plasma Panel (Exiqon). From 241 ratios differently expressed between cancer patients and healthy individuals 19 miRNAs were selected for verification using the same platform. LASSO-penalized logistic regression model, including 10 miRNA ratios comprised of 14 individual miRNAs discriminated lung cancer patients from both control groups with AUC of 0.979

Long interspersed nuclear element-1 methylation status in the circulating DNA from blood of patients with malignant and chronic inflammatory lung diseases

Along with other malignant diseases, lung cancer arises from the precancerous lung tissue state. Aberrant DNA methylation (hypermethylation of certain genes and hypomethylation of retrotransposons) is known as one of the driving forces of malignant cell transformation. Epigenetic changes were shown to be detectable in DNA, circulating in the blood (cirDNA) of cancer patients, indicating the possibility to use them as cancer markers. The current study is the first to compare the Long interspersed nuclear element-1 (LINE-1) methylation level in the blood from lung cancer patients before treatment versus different control groups as healthy subjects, patients with bronchitis and patients with chronic obstructive pulmonary disease (COPD). The concentration of LINE-1 methylated fragments, region 1 (LINE-1 methylated, LINE-1-met) was estimated by quantitative methyl-specific PCR. The total concentration of the circulating LINE-1 copies was measured by qPCR specific for LINE-1 region 2, which was selected due to its CpG methylation-independent sequence (LINE-1-Ind). Both LINE-1 methylation level and LINE-1 methylation index (LINE-1-met/LINE-1-Ind ratio) was decreased in lung cancer patients compared with the joint control group (healthy subjects + patients with bronchitis + COPD patients) (Mann-Whitney U-test, P = 0.016). We also found that the tendency of LINE-1 methylation index decreases in the cirDNA from lung cancer patients versus COPD patients (Mann-Whitney U-test, P = 0.07). Our data indicate that the quantitative analysis of the LINE-1 methylation level in the cirDNA is valuable for discrimination of lung cancer patients from patients with chronic inflammatory lung diseases. © 2021 Lippincott Williams and Wilkins. All rights reserved

Plasma miR-19b and miR-183 as Potential Biomarkers of Lung Cancer

<div><p>Lung cancer is a complex disease that often manifests at the point when treatment is not effective. Introduction of blood-based complementary diagnostics using molecular markers may enhance early detection of this disease and help reduce the burden of lung cancer. Here we evaluated the diagnostic potential of seven plasma miRNA biomarkers (miR-21, -19b, -126, -25, -205, -183, -125b) by quantitative reverse transcription PCR. Influence clinical and demographical characteristics, including age, tumor stage and cancer subtype on miRNA levels was investigated. Four miRNAs were significantly dysregulated (miR-19b, -21, -25, -183) in lung cancer patients. Combination of miR-19b and miR-183 provided detection of lung cancer with 94.7% sensitivity and 95.2% specificity (AUC = 0.990). Thus, miRNAs have shown the potential to discriminate histological subtypes of lung cancer and reliably distinguish lung cancer patients from healthy individuals.</p></div

Dynamic changes in circulating miRNA levels in response to antitumor therapy of lung cancer

<p><b>Purpose:</b> Expression levels of cancer-associated microRNAs were reported to be altered in serum/plasma samples from lung cancer patients compared with healthy subjects. The purpose of this study was to estimate the value of five selected miRNAs plasma levels as markers of response to antitumor therapy in lung cancer patients. <b>Materials and Methods:</b> Expression levels of miR-19b, miR-126, miR-25, miR-205, and miR-125b have been evaluated by quantitative reverse transcription PCR versus control miR-16 in blood plasma samples from 23 lung cancer (LC) patients. Plasma samples were obtained from LC patients before treatment (untreated-UT), within 30 days after completing two courses of chemotherapy (postchemotherapy-PC) and 15 days after surgery (postoperative-PO). <b>Results:</b> Repeated Measures ANOVA demonstrated that miR-19b expression levels were decreased in PC and increased in PO samples. These changes were characterized by a significant quadratic trend (<i>p</i> = 0.03). Expression levels of miR-125b increased both after chemotherapy and again after surgery and demonstrated a significant linear trend (<i>p</i> = 0.03). The miR-125b/miR-19b ratio changed during the course of the antitumor treatment with a significant linear trend (<i>p</i> = 0.04). Individual analysis in the groups of patients with partial response to chemotherapy and patients with stable or progressive disease showed different trends for miR-19b, miR-125b, and miR-125b/miR-19b ratio between the groups. The Kaplan–Meier survival curves demonstrated an association of miR-125b/miR-19b ratio value with the survival time without the tumor relapse (<i>p</i> < 0.1). <b>Conclusions:</b> Dynamic change of trends for miR-19b and miR-125b expression levels and miR-125b/miR-19b ratio in the blood plasma have shown a potentiality to discriminate types of response to antitumor therapy in lung cancer patients. Further in-depth investigation is needed to establish a direct link the miRNAs expression levels in blood plasma with therapy response and patient's survival.</p

ROC analysis of miRNA expression.

<p>Receiving Operator Characteristic (ROC) curves for individual miRNAs and combination of miR-19b and miR-183. (A)–(E) Individual miRNAs; (F) Binary logistic regression of miR-19b and miR-183. ROC curves discriminate squamous cell carcinoma (SCC), adenocarcinoma (AD) and total study population of lung cancer patients (LC) from healthy individuals (HD).</p